Table S1.

Load-independent hemodynamic measures

Parameter	WT (n = 11)	β-arrestin 1 KO (n = 4)	β-arrestin 2 KO (n = 6)	AT1R KO (n = 13)
Compliance parameters
EDPVR (linear) slope	0.26 ± 0.05	0.17 ± 0.01	0.21 ± 0.02	0.35 ± 0.04
EDPVR (linear) intercept	−3.06 ± 1.34	−1.46 ± 1.72	−2.45 ± 1.26	−7.74 ± 2.67
EDPVR (quadratic) β coefficient	0.083 ± 0.015	0.038 ± 0.010	0.115 ± 0.041	0.047 ± 0.008
EDPVR (quadratic) α coefficient	1.164 ± 0.445	1.974 ± 0.992	1.070 ± 0.563	1.260 ± 0.554
Contractility parameters
ESPVR (linear) slope	4.02 ± 0.68	3.19 ± 0.29	3.48 ± 0.91	2.68 ± 0.26
ESPVR (linear) intercept	−4.53 ± 7.39	−6.60 ± 7.59	−7.19 ± 10.55	−12.12 ± 6.71
ESPVR (quadratic) a	−1.82 ± 1.14	−0.61 ± 0.25	−0.34 ± 0.12	−0.04 ± 0.01*
ESPVR (quadratic) V0	9.85 ± 5.80	4.70 ± 7.00	9.47 ± 4.95	−0.90 ± 5.68
ESPVR (quadratic) E′max	16.09 ± 3.84	11.59 ± 2.24	9.93 ± 1.93	5.02 ± 0.55*
PRSW slope	59.69 ± 4.11	59.26 ± 4.91	42.63 ± 7.86	44.46 ± 4.83
PRSW intercept	8.10 ± 6.94	9.88 ± 4.53	1.87 ± 8.70	−0.92 ± 3.68
dP/dtmax vs. EDV slope	154.50 ± 22.74	103.50 ± 9.78	88.09 ± 10.21	113.40 ± 17.71
dP/dtmax vs. EDV intercept	−14.59 ± 8.39	−19.63 ± 5.16	−32.39 ± 11.67	−30.26 ± 13.79
Emax	7.79 ± 1.29	8.29 ± 1.11	6.44 ± 1.41	4.80 ± 0.47

Average load-independent parameters obtained by inferior vena cava constriction performed on a separate cohort of WT (n = 11), β-arrestin 1 KO (n = 4), β-arrestin 2 KO (n = 6), and AT1R KO (n = 7) mice. Parameters of LV compliance (linear- and quadratic-derived EDPVR) and LV contractility (linear- and quadratic-derived ESPVR, PRSW, dP/dt_max vs. EDV and E_max) are listed separately for each genotype. Errors reflect SEM. ESPVR, end-systolic PV relationship; a, coefficient of curvilinearity; V_o, volume intercept; E′_max, maximum slope of quadratic ESPVR; PRSW, preload recruitable stroke work; E_max, maximal elastance.

^*P < 0.05 vs. WT, one-way ANOVA with Bonferroni’s multiple comparison test.