Table 1.
GO analysis of observed Tajima’s D values uncovers evidence for enrichment of GO terms in genes under strong directional and balancing selection
| Dataset* | Feature† | D percentile‡ | GO term | GO ID | P value§ |
| P. vivax (M/A) | Exon | First | Histone deacetylase complex | GO:0000118 | 0.0423 |
| P. vivax (M) | Gene | First | ATP binding | GO:0005524 | 0.0493 |
| P. vivax (A) | Gene | First | Cellular component | GO:0005575 | 0.00941 |
| P. vivax (M) | Exon | 99th | Metallo-sulfur cluster assembly | GO:0031163 | 0.0440 |
| P. vivax (M) | Exon | 99th | Iron-sulfur cluster assembly | GO:0016226 | 0.04402 |
| P. vivax (M/A) | Gene/exon | 99th | Plastid large ribosomal subunit | GO:0000311 | 0.0166 |
| P. vivax (M/A) | Gene/exon | 99th | Plastid ribosome | GO:0009547 | 0.0306 |
| P. vivax (M/A) | Gene/exon | 99th | Plastid part | GO:0044435 | 0.0306 |
| P. vivax (M/A) | Gene/exon | 99th | Plastid stroma | GO:0009532 | 0.0306 |
| P. falciparum | Gene | First | Retrograde transport, endosome to Golgi | GO:0042147 | 0.0431 |
| P. falciparum | Gene | First | Endosomal transport | GO:0016197 | 0.0431 |
| P. falciparum | Gene/exon | 99th | Cell surface | GO:0009986 | 0.00124 |
Tajima’s D was calculated on a per-gene and per-exon basis for the entire P. vivax population sample, the P. vivax monoclonal subset, and the central, ancestral-like P. falciparum subpopulation. For genewise and exonwise Tajima’s D values, the first percentile (largest negative values) and 99th percentile (largest positive values) were included in GO term enrichment analysis.
(M) indicates the result was found among P. vivax monoclonals; (A) indicates result found among the entire P. vivax population sample.
GO-term enrichment was found in exonwise analysis, genewise analysis, or both exon- and genewise analyses.
First percentile indicates largest negative Tajima’s D values, consistent with directional selection; 99th percentile indicates largest positive Tajima’s D values, consistent with balancing selection.
Bonferroni-corrected P values; if a term was significant (to a Bonferroni-corrected P ≤ 0.05) in both monoclonal (M) and all (A) analyses, the more conservative (higher) of the two P values is reported.