Vangl2 was efficiently deleted in uteri of Vangl2d/d mice. (A–C) Western blotting (A), qPCR (B), and IF (C) of Vangl2 in day 4 uteri of Vangl2f/f and Vangl2d/d mice. (D–F) Western blotting (D), qPCR (E), and IF (F) of Vangl2 in day 5 implantation sites of Vangl2f/f and Vangl2d/d mice. (Scale bars, 100 µm.) Asterisks indicate the location of the embryo. (G) Results of Western blotting show that stromal Vangl2 is reduced in Vangl2d/d mice.