Fig. S4.
Vangl2 interacts with Scrib in the uterus. (A) The BL21 strain containing GST-ScribPDZ3 plasmid which generated Scribe PDZ3 domain fusion protein with GST tag was cultured in LB medium for GST pull-down assay. GST pull-down assays were performed using a standard protocol. The results shows Scrib can pull down Vangl2 in uterus. RIPA buffer, radioimmunoprecipitation assay buffer. (B) Spatiotemporal localization of Vangl2 and Scrib are first distinctly expressed in the LE and targeted to the apical surface on day 3 of pregnancy. IF of Vangl2 and Scrib from days 1 through 3 of pregnancy. (Scale bars, 100 μm.)