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. 2016 Dec 15;10(1):80–89. doi: 10.1016/j.tranon.2016.11.004

Figure 4.

Figure 4

CCND1 and USP28 are direct targets of miR-3940-5p. (A and B) Specific locations of the binding sites in miR-3940-5p and 3′UTRs of CCND1 or USP28 are marked. (C and D) The luciferase reporter assay shows that miR-3940-5p significantly suppresses the luciferase activity of reporters containing the WT 3′UTRs of CCND1 or USP28 but has no significant effect on reporters with MUT 3′UTRs. The luciferase activity of each sample is normalized to Renilla luciferase activity. (E and F) Decreased expression of CCND1 or USP28 mRNA is due to miR-3940-5p overexpression in A549 cells. (G and H) The restoration of miR-3940-5p obviously inhibits protein levels of CCND1 and USP28. β-Actin is shown as a loading control. *P < .05.