Fig 3. Droplet cluster with digested DNA, different probe concentrations, dyes and quenchers.

ddPCR with EGDe DNA (≈ 10³ copies/sample) shows that the rain is similar when DNA is digested with BamHI, higher or lower concentrations of the probe were applied, FAM (blue, Ch1 amplitude) or VIC (green, Ch2 amplitude) as dyes were used, MGB probes (all but “BHQ1”) or probes from another supplier were introduced. Unless indicated otherwise, 312.5 nM MGB probe with FAM as dye and the non-fluorescent quencher were used. For optimal comparison of quenchers and dyes, the last three probes were ordered and tested simultaneously.