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. 2016 Dec 19;11(12):e0168040. doi: 10.1371/journal.pone.0168040

Fig 4. Overexpression of PtPYRL1 enhanced ABA sensitivity in the transgenic Arabidopsis plants.

Fig 4

(A) Expression level of PtPYRL1 in wild type (WT) and transgenic lines (L1-32, L1-17, L1-15, L1-11 and L1-6) grown at MS for 20 days. The level was detected using gene-specific primers after 26 PCR cycles. ACTIN2 was used as endogenous control in all cDNA samples. (B) qRT-PCR analysis on PYRL1 expression in wild type and transgenic seedlings. Total RNA was isolated from 20-day-old whole seedlings grown at MS. The relative expression level was calculated as the ratio of PtPYRL1 level to ACTIN2 level. (C) Representative images of 8-day-old wild type (WT) and transgenic lines (L1-32, L1-17, and L1-6) grown at the MS medium without (upper panel) or with 1μM ABA (low panel). The germination percentage of the seeds of wild type (WT) and transgenic lines (L1-32, L1-17, and L1-6) during growth at the MS medium containing 0 ABA (D), 1 μM ABA (E), or 2 μM ABA (F). Approximately 200 seeds were used in each experiment, and each experiment was repeated three times. Data are the standard deviation and significant differences are indicated as **P < 0.01 and ***P < 0.001 (ANOVA test).