Fig 5. Overexpression of PtPRYL1 in Arabidopsis conferred drought resistance.
(A) Representative images of wild type and transgenic PtPRYL1 lines under drought stress. The wild type (WT) and transgenic lines (L1-32, L1-17 and L1-6) were grown in soil with sufficient water for 10 days (Watered) (upper panel), and then water was withheld for 20 days before re-watered (Drought) (middle panel). Plants were then allowed to recover by watered again for 3 d before taking pictures (Re-watered) (low panel). (B) Quantification of the survival rate of the wild type (WT) and transgenic lines (L1-32, L1-17 and L1-6) at the third day after rewatering. Survival rates and standard deviations were calculated from the results of three independent experiments. (C) Quantification of shoot weight of the wild type (WT) and transgenic lines (L1-32, L1-17 and L1-6) at the third day after rewatering. Values are means ± SD (n = 26). Proline content (D) and MDA level (E) in the wild type (WT) and transgenic lines (L1-32, L1-17 and L1-6) after drought treatment for 20 days. Results are presented as means ± SD from three independent experiments.