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. 2016 Oct 26;17(12):1814–1828. doi: 10.15252/embr.201642395

Figure 1. General outline of the experimental strategy.

Figure 1

  1. Developmental stages of ESC differentiation to HSPCs, showing the populations isolated on days 6 and 20.
  2. Schematic overview of the experimental strategy.
  3. FACS plots from a representative experiment showing gates for purification of the cell populations. Upper left: GFP+ cells in the population of ESCs infected with pGIPZ‐shRNA library lentiviruses. Upper right: Isolation of mesodermal (SSEA1−Flk1+Cxcr4−) and endodermal (SSEA1−Flkl−Cxcr4+) cells. Lower panels: Isolation of D20LS (Lin−Sca1+c‐Kit−), D20LK (Lin−Sca1−c‐Kit+), and D20LSK (Lin−Sca1+c‐Kit+) cells.