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. 2016 Oct 26;17(12):1814–1828. doi: 10.15252/embr.201642395

Figure 2. Confirmation of the identity of the sorted cell populations.

Figure 2

  1. mRNA analysis of expression of endothelial (Tie2, CD31, Cdh5), epithelial (Cdh1), and early hematopoietic (Scl, CD41) marker genes in the indicated sorted cell populations. Error bars represent standard deviation (SD), n = 3. Student's t‐test. *P < 0.05.
  2. Confirmation of the purity of sorted populations by qRT–PCR: c‐Kit is highly expressed only in the c‐Kit+ (D20LSK and D20LK) populations, and Sca1 is highly expressed only in the in Sca1+ (D20LSK and D20LS) populations. Error bars represent standard deviation (SD), n = 3. Student's t‐test. *P < 0.05.
  3. qRT–PCR analysis of Gata1, Gata2, Lyz2, and Scl expression in the sorted cell populations on day 20. Error bars represent standard deviation (SD), n = 3. Student's t‐test. *P < 0.05.
  4. FACS analysis of ESC‐derived cells confirming that D20LS, D20LK, and D20LSK cell populations express the early hematopoietic marker CD41 and the pan‐hematopoietic marker CD45.