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. 2016 Oct 26;17(12):1814–1828. doi: 10.15252/embr.201642395

Figure 3. Distinct groups of shRNA target genes are enriched or diminished in different cell populations.

Figure 3

  1. Clustering and enrichment analyses of shRNA reads in D6C (endoderm), D6F (HM/E), D20LS, D20LK, and D20LSK (HSPC) populations compared with ESCs. Specifically enriched/depleted shRNAs are grouped according to their target gene function (I–X). Color coding bar is shown below.
  2. Heat map of shRNA reads in D6C and D6F populations compared with ESCs. shRNAs specifically depleted in D6C and D6F are labeled as Groups XI and XII, respectively.
  3. GO pathway analysis showing the top 5 enriched biological processes specific to D6F (XII), D20LS (VI), D20LK (V), and D20LSK (X) populations. The enrichment analysis was conducted using the Metacore platform.
  4. GO analysis showing several key pathways, including Wnt signaling, cell adhesion, and cell cycle regulation, that are important at all stages of HSPC development.
  5. Heat map showing depletion of shRNAs targeting Cdc and Cdk genes in all day 20 samples.