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. 2016 Oct 26;17(12):1814–1828. doi: 10.15252/embr.201642395

Figure EV3. Knockdown of Group XII, XI, IV, and X genes and analysis of their function in differentiation.

Figure EV3

  1. qRT–PCR analysis showing the knockdown of Group XII genes Esrra, S100a8, and Hcfc2 in mESCs. Error bars represent standard deviation (SD) of technical triplicates. Student's t‐test, *P < 0.05.
  2. qRT–PCR analysis showing the knockdown of Group XI genes Atp5g3, Bub1b, Eci1, and Dis3l in mESCs. Error bars represent standard deviation (SD) of technical triplicates. Student's t‐test, *P < 0.05.
  3. qRT–PCR analysis showing the knockdown of Group IV genes Wbp5, Rbm26, Gdpd4, and Nrxn1 in mESCs. Error bars represent standard deviation (SD) of technical triplicates. Student's t‐test, *P < 0.05.
  4. Flow cytometric analysis of endoderm and mesoderm/endothelium cells in EBs on day 6 with knockdown of Wbp5, Rbm26, Gdpd4, and Nrxn1, respectively.
  5. Percentage of CXCR4+ cells in EBs on day 6 with knockdown of Wbp5, Rbm26, Gdpd4, and Nrxn1. Error bars represent standard deviation (SD), n = 2. Student's t‐test, *P < 0.05.
  6. Percentage of Flk1+ cells in EBs on day 6 with knockdown of Wbp5, Rbm26, Gdpd4, and Nrxn1. Error bars represent standard deviation (SD), n = 2. Student's t‐test shows no significance.
  7. qRT–PCR analysis showing the knockdown of Group X genes Ap2a1, Mettl22, Lrsam1, and Hal in mESCs. Error bars represent standard deviation (SD) of technical triplicates. Student's t‐test, *P < 0.05.
  8. Flow cytometric analysis of endoderm and mesoderm/endothelium cells in EBs on day 6 with knockdown of Ap2a1, Mettl22, Lrsam1, and Hal.
  9. Percentage of CXCR4+ cells in EBs on day 6 with knockdown of Ap2a1, Mettl22, Lrsam1, and Hal. Error bars represent standard deviation (SD), n = 3. Student's t‐test shows no significance.
  10. Percentage of Flk1+ cells at day 6 with knockdown of Ap2a1, Mettl22, Lrsam1, and Hal. Error bars represent standard deviation (SD), n = 3. Student's t‐test shows no significance.
  11. qRT–PCR analysis showing the knockdown AP2A1, METTL22, LRSAM1, and HAL in human CD34+ cells. Error bars represent standard deviation (SD) of technical triplicates. Student's t‐test, *P < 0.05.