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. 2016 Nov 23;3(5):467–479.e12. doi: 10.1016/j.cels.2016.10.012

Quartz-seq enrichment PCR where xx is the chosen second-strand synthesis temperature of 42 or 72

Cycle Temperature (°C) Time
1 68 To begin
2-18 98 10 s
2-18 65 15 s
2-18 xx 5 min
19 xx 5 min
20 4 Infinite hold