Fig 2. The effects of BBP and MEHP on mRNA translation in HEK-293T cells.

HEK-293T cells were grown to 80% confluence and transfected with luciferase reporter. Eight hours later, cells were treated with BBP or MEHP for 24 hrs at the concentrations indicated. Equal volume of vehicle (methanol) was used as the control. Cells were harvested by washing once with cold PBS, and the cell lysate was prepared with PBL buffer (Promega) for the luciferase activity measurement using dual luciferase assay kit (Promega). (A) Schematic diagram of the reporter construct used in this experiment and throughout the whole study. (B) The effect of BBP on cap-dependent and IRES-driven translation; luciferase activity is the readout of translational activity. (C) The effect of MEHP on cap-dependent and IRES-driven translation. The structure of MEHP was inserted in the chart. The experiments were repeated three times, and the means were shown with standard deviations.