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. 2016 Dec 19;11(12):e0167914. doi: 10.1371/journal.pone.0167914

Fig 5. Measurement of binding of BBP to eIF4E by SPR.

Fig 5

Purified eIF4E was immobilized to the Fc2 channel of a CM5 sensor chip as the ligand. The immobilization was performed through amine-coupling reaction. Fc1 channel was treated similarly but without protein as the control. Different concentrations of BBP were applied through Fc1 and Fc2 channels as the analyte to measure the binding. (A) Adjusted sensorgrams (Fc2-Fc1) showing the binding of eIF4E to various concentrations of BBP. (B) Calculation of binding affinity using steady-state fitting model.