FIG. 1.

FOXO3a regulates ERα expression. (A) Samples (40 μg) of WCE or nuclear extracts (NE) from the indicated cell lines were analyzed by immunoblotting for FOXO3a (FOX), ERα, or β-actin (actin) as control for equal loading. (B) NF639 cells were transfected with 8 μg of pECE EV, WT FOXO3a, or A3 FOXO3a. Forty-eight hours after transfection, WCE were analyzed by immunoblotting for ERα, FOXO3a, and β-actin. (C) MCF-7 cells were infected with an increasing multiplicity of infection (MOI) of dnFOXO3a-expressing adenovirus. After 40 h, WCE were prepared and samples (40 μg) were analyzed by immunoblotting for FOXO3a and ERα. Equal loading was verified using β-actin immunoblotting and Ponceau S (Ponc.) staining.