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. 2016 Dec 19;11(12):e0168080. doi: 10.1371/journal.pone.0168080

Fig 5. Time-course of IL-33 expression in the ligature-induced murine model of experimental periodontitis.

Fig 5

A. IL-33 expression was assessed by IHC and sections were counterstained with Harris Hematoxylin staining (arrows). B. The percentage of IL-33 was quantified in gingival epithelium and in connective tissue using Fiji software and defined as a percentage of DAB positive staining area per region of interest. At each time point, data of ligatured groups (Lig and Pg L) were compared to their respective Sham groups. EP: Epithelium, CT: Connective tissue. Data are shown as means ± SEM. * p<0.05; **p<0.01. Scale bar = 100μm.