FIG. 5.

Importance of c-CRD for the nuclear localization of Yap1 following MG treatment. (A) Schematic of Yap1 and the Gal4-GFP-c-CRD reporter protein. (B) The Gal4-GFP-c-CRD reporter gene was expressed in wild-type (WT) and glo1Δ cells. Cells were cultured in SD medium to an A₆₁₀ of 0.5, and localization of the reporter protein was monitored. Then 8 mM MG was added, and the cells were incubated for 60 min, at which time the localization of the reporter protein was again determined.