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Picrosirius Red Quantification. The brightness analysis revealed an average greyscale brightness of 83.63 ±24.90 for native ACL, 12.88 ±8.08 brightness at the 3-day time point, 17.08 ±12.65 brightness at the 7-day time point, 21.60 ±6.82 brightness at the 14-day time point, 26.21 ± 7.20 brightness at the 28-day time point, and 24.64 ± 3.12 brightness for the acellularized group. Construct collagen alignment at all time points was significantly less than native ligament. (P<0.05).
