Table 1.

Detection of male cells by Y chromosome PCR analysis.

	# of BLBs that contain male DNA
3-Day	2/3*
7-Day	3/4
14-Day	3/4
28-Day	2/4
Acellularized	0/3

BLB constructs were engineered from bone marrow derived mesenchymal stem cells (MSCs) obtained from male donor sheep. There was significant amplification of the Y-chromosome specific sequence Ucd043 in both the pre-implant and 3-day explant samples. Three sections of each BLB were tested: BLB spanning intracapsular space, BLB found within the femoral bone tunnel, and BLB found within the tibial bone tunnel. In the PCR results, if any of these three sections showed detectable levels of male DNA, that particular BLB was considered positive for male sheep DNA. The numerators represent the number of BLBs containing male DNA, and the denominators are the total number of successfully tested BLBs for each group.

^*Insufficient DNA for PCR for one of the 3-day samples.