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. 2004 Oct;24(19):8332–8341. doi: 10.1128/MCB.24.19.8332-8341.2004

FIG. 1.

FIG. 1.

Individual Sap proteins play distinct roles in Tor signaling. Isogenic wild-type (WT; MLY41a), sit4 (SCY94), no-SAP (sap4 sap155 sap185 sap190; JRY40), only-SAP4 (sap155 sap185 sap190; JRY45), only-SAP185 (sap4 sap155 sap190; JRY43), only-SAP190 (sap4 sap155 sap185; JRY44), tip41 (JRY39), only-SAP155 (sap4 sap185 sap190; JRY46), sap185 (JRY20), sap190 (JRY21), and sap185 sap190 (JRY29) strains were grown overnight at 30°C in YEPD. Equivalent numbers of cells were serially diluted, and aliquots were spotted onto plates of YEPD or YEPD containing 0, 20, or 100 nM rapamycin (rapa). After 3 days of incubation at 30°C, the plates were photographed.