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. 2004 Oct;24(19):8332–8341. doi: 10.1128/MCB.24.19.8332-8341.2004

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Copyright © 2004, American Society for Microbiology

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FIG. 2. — The Sap products are required for proper induction of a subset of Tor-regulated genes. (A) Exponentially growing cultures of isogenic wild-type (JRY32), no-SAP (sap4 sap155 sap185 sap190; JRY40), only-SAP155 (sap4 sap185 sap190; JRY46), only-SAP185 (sap4 sap155 sap190; JRY43), only-SAP190 (sap4 sap155 sap185; JRY44), sit4 (SCY94), tip41 (JRY39), sap185 sap190 (JRY29), and only-SAP4 (sap155 sap185 sap190; JRY45) strains were treated with 100 nM rapamycin for 0, 15, 60, or 120 min. RNA was prepared and analyzed by Northern blotting with radioactive probes that hybridize to the genes indicated at the right. (B) Northern blot signals for the RP genes (RPG) and MEP2 were quantified and normalized to the ACT1 loading control. Relative expression values for the two RP genes examined were averaged. The results shown in the graphs are the percentage of gene expression, with the time zero value as 100% (for the RP genes) or the strain with the maximal level of expression at 15 min as 100% (for MEP2).