FIG. 3.

Rapamycin-induced activation of Gcn4 target genes requires Gcn2. Exponentially growing cultures of isogenic wild-type (MLY41), sap185 sap190 (JRY29), sap185 sap190 gcn2 (JRY48), and gcn4 gln3 (JRY49) mutant strains were treated with 100 nM rapamycin for 0, 15, or 60 min. RNA was prepared and analyzed by Northern blotting with radioactive probes that hybridize to the genes indicated at the left. The Northern blot specific signal for MEP2 was quantified as indicated in the Fig. 2 legend. The results are presented in the graph as the percentage of gene expression, assigning as 100% the expression level detected at 15 min.