Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
The sap185 sap190 mutant strain exhibits Gcn2-dependent rapamycin-hypersensitive translation. (A) Polysome traces from isogenic wild-type (MLY41) and mutant strains sap185 sap190 (JRY29) and sap185 sap190 gcn2 (JRY48). Strains were grown in YEPD medium, and either 200 nM rapamycin or drug vehicle (DV) was added for the indicated time periods. Polysomes were analyzed as described in Materials and Methods. The P/M ratio for each strain is plotted in panel B.
