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Following knockdown of IKKα in HeLa (A) and U5A cells (B), poly I:C was introduced for an additional 4 h and 10 h, respectively. HeLa cells were treated with IFN-α (10 ng/ml) for 20 min to confirm phosphorylation of JAK1 and Tyk2 as a positive control. Cell extracts were analyzed by immunoblotting. The results are representative of three independent experiments.
