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. 2016 Dec 19;11(12):e0168562. doi: 10.1371/journal.pone.0168562

Fig 4. Differentiation-dependent expression and release of CXCL2 in 3T3-L1 cells.

Fig 4

NIH 3T3 fibroblasts and 3T3-L1 pre-adipocytes were cultured in differentiation medium (DIFF) or remained undifferentiated (ND) in control medium (DMEM) for 12 days. At day 12, differentiated (DIFF) and non-differentiated (ND) 3T3-L1 cells were stimulated with cytokines (25 ng/ml IL-1β, 50 ng/ml TNFα) as indicated. NIH 3T3 fibroblasts served as a control. CXCL2 mRNA expression was analyzed by qRT-PCR (a). CXCL2 protein release into cell culture supernatants was determined by ELISA (b). **, p < 0.01; (Student’s unpaired t test) compared to non-differentiated cells. Bars indicate the mean ± S.D. obtained from four independent experiments (n = 4).