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. 2016 Dec 19;11(12):e0168562. doi: 10.1371/journal.pone.0168562

Fig 5. MAPK signaling in cytokine-stimulated differentiated and non-differentiated 3T3-L1 cells.

Fig 5

3T3-L1 pre-adipocytes were differentiated in DMI and DMII for 12 days or remained non-differentiated in basal medium I. Differentiated and non-differentiated 3T3-L1 cells were stimulated with cytokines (25 ng/ml IL-1β, 50 ng/ml TNFα) as indicated. Cell lysates were subsequently analyzed by immunoblot for the presence of phosphorylated p42/44 MAPK (a), degradation of IκBα (b) and phosphorylation of SAPK/JNK (c) as indicated. Total p42/44 (a, lower panel), β-actin (b, lower panel) or total SAPK/JNK (c, lower panel) served as loading controls.