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. 2016 Dec 19;11(12):e0168562. doi: 10.1371/journal.pone.0168562

Fig 6. Key transcription factors of adipogenesis are increased in subcutaneous fat of obese mice.

Fig 6

qRT-PCR quantification of KLF5 (a), PPARγ1 (b) and PPARγ2 (c) mRNA expression in subcutaneous fat tissue of wildtype and obese (ob/ob) mice as indicated. *, p < 0.05 (Student’s unpaired t test) as compared to wildtype mice. Bars indicate the mean ± S.D. obtained from fat tissue isolated from four individual animals (n = 4). Subcutaneous fat was analyzed by immunoblot for the presence of KLF5, PPARγ1 and PPARγ2 protein as indicated (d). Lysates from differentiated 3T3-L1 adipocytes served as a positive control for KLF5 and PPAR-specific immunoblot signals. β-actin was used to control loading.