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. 2016 Dec 19;11(12):e0168562. doi: 10.1371/journal.pone.0168562

Fig 9. PPARγ-agonist rosiglitazone reduces Cox-2 expression in finally differentiated 3T3-L1 adipocytes.

Fig 9

3T3-L1 pre-adipocytes remained non-differentiated in non-differentiating control medium (ND) or were stimulated to induce the differentiation process in differentiating medium (DIFF) for 4 days or 12 days as indicated. 3T3-L1 cells in non-differentiating medium (ND) (a) or differentiating medium (DIFF) (b) were then stimulated with cytokines (25 ng/ml IL-1β, 50 ng/ml TNFα) for 8 h in the presence of increasing concentrations of rosiglitazone (1–4 μM) before (day 4) and after (day 12) maturation to adipocytes. Cox-2 mRNA expression was analyzed by qRT-PCR. *, p < 0.05; n.s., not significant (Student’s unpaired t test) compared to cytokine-treated, but rosiglitazone-free cells. Bars indicate the mean ± S.D. obtained from four independent experiments (n = 4).