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. 2016 Dec 19;11(12):e0168562. doi: 10.1371/journal.pone.0168562

Fig 10. Effects of rosiglitazone on SAPK/JNK and NF-κB signaling in differentiated 3T3-L1 cells.

Fig 10

3T3-L1 pre-adipocytes were differentiated in DMI and DMII for 12 days. Differentiated 3T3-L1 cells were stimulated with cytokines (25 ng/ml IL-1β, 50 ng/ml TNFα) in the presence or absence of rosiglitazone (4μM) as indicated. Cell lysates were subsequently analyzed by immunoblot for the presence of phosphorylated SAPK/JNK (a), the phosphorylation of the NF-κb p65 subunit (b) and degradation of IκBα (d). Total NF-κb p65 (c) and GAPDH (e) served as loading controls.