Shown are methylated cytosine (mC) states (0 = no methylation; 1 = fully methylated sequence area) in differentially methylated regions (DMRs) plotted against normalized gene expression (y-axis) of leaf (a) and root (c) material from clonal Populus trichocarpa (cv. Muhle Larson) cuttings derived from two different short rotation forestry sites (Anderlingen vs. Wallstawe), grown under optimal (+P) conditions. Additionally, quantification of miRNA/small RNA ratio in total RNA samples of leaf (c) and root (d) material from the same clonal Populus trichocarpa cuttings, grown under adequate (+P) and deficient (−P) phosphorus supply, are illustrated. Data are presented as the mean ± SE, p ≤ 0.05 and 95% confidence intervals (c,d) and were obtained from 3 independent experiments.