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. 2016 Dec 19;11(12):e0168623. doi: 10.1371/journal.pone.0168623

Fig 7. Target gene expression differences of clonal Populus trichocarpa material.

Fig 7

qPCR results from Populus trichocarpa (cv. Muhle Larson) root material derived from two different short rotation forestry sites (Anderlingen (a,c) and Wallstawe (b,d)), grown under controlled adequate (+P) vs. deficient (−P) phosphorus nutrition using 3 reference genes (POPTR_EF103B0031, POPTR_RP and POPTR_18s) for normalization. Normalized gene expression (y-axis) is shown for differentially methylated miRNAs (a,b) and their possible target genes (c,d): Ptc-miR1446ae, Ptc-miR481ab, Ptc-miR4b1cd, Ptc-miR6432, Ptc-miR827, POPTR_0004s02320 as Pt04s02320, POPTR_0006s04360 as Pt04s04360, POPTR_0006s09220 as Pt06s09220 and POPTR_0013s14900 as Pt13s14900 (x-axis). Black triangles indicate which plant material had a higher methylation level. Data are presented as the mean ± SEM, p* ≤ 0.05 and 95% confidence intervals and were obtained from 3 independent experiments.