FIG. 5.

Endoplasmic reticulum or oxidative stress stabilizes Nrf2. (a) 293T cells were mock treated (lane 1), treated with 100 μM tBHQ for 8 h (lane 2), or treated with 5 μg of tunicamycin (Tun) (lane 3) per ml for 1 h. Total Nrf2 levels were detected via immunoprecipitation followed by immunoblot analysis. Lane 4 shows a control precipitation with a nonspecific antiserum. (b) NIH 3T3 cells were treated for 30 min with dimethyl sulfoxide (DMSO) (lanes 2 to 5) or with 5 μg of tunicamycin per ml (lanes 6 to 9) before the addition of 10 μM cycloheximide (CHX) for the indicated intervals. Nrf2 was detected by immunoprecipitation followed by immunoblot analysis. Lane 1 shows a control precipitation with a nonspecific antiserum.