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. Author manuscript; available in PMC: 2017 Mar 29.
Published in final edited form as: Biochemistry. 2016 Mar 16;55(12):1909–1917. doi: 10.1021/acs.biochem.6b00096

Figure 4.

Figure 4

ERK2 binds multiple oligonucleotides. (A) Gel shift assay with ERK2 and activated doubly phosphorylated ERK2 with radiolabeled sequences from GH1, ULK1, and IGFBP2 genes. (B) Gel shift assays with ERK2 and ppERK2 and a trace amount of fluorescently labeled oligonucleotide (100 pM) that maps to the insulin promoter and contains the motif GAAAC. (C) Dephosphorylation with protein phosphatases PTP1 (phosphotyrosine-selective) and PP2A (phosphoserine/threonine-selective) reduces the interaction between ppERK2 and the GH1 oligonucleotide. (D) Gel shift assays with a trace amount of fluorescently labeled oligonucleotide (mIns19) (100 pM) with T188D and T188V.