FIG.4.
Mapping the region of myocardin that interacts with GATA4. (A) A GST-GATA4 fusion protein encompassing amino acids 177 to 332 was incubated with [35S]methionine-labeled myocardin proteins translated in vitro. Myocardin proteins associated with GST-GATA4 are shown at the top, and 10% of the input proteins are shown at the bottom. (B) Summary of the GST-GATA4 pulldown and coimmunoprecipitation assays (data not shown). (C) GST pulldown assays were performed with the indicated myocardin mutants and GST-GATA4 as described for panel A. (D) Summary of the GST-GATA4 pulldown assays in panel C.