Renal tissue from sham, vehicle, IgG control, and anti-OPN Ab treated mice were collected to measure levels of cytokines (A) IL-6 and (B) TNF-α by ELISA. Data are expressed as mean ± SEM (n=6–9 mice/group) and compared by one-way ANOVA by SNK method. *p<0.05 vs. sham; #p<0.05 vs. vehicle; ψp<0.05 vs. IgG. OPN, osteopontin; IL, interleukin; TNF, tumor necrosis factor; ELISA, enzyme-linked immunosorbent assay.