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. 2004 Oct;24(19):8778–8789. doi: 10.1128/MCB.24.19.8778-8789.2004

FIG. 2.

FIG. 2.

Expression of small t antigen does not affect tyrosine phosphorylation of IRS-1 or its association with PI 3-kinase. (A) 3T3-L1 adipocytes infected with either control virus (C) or small-t-antigen-encoding adenovirus (S) were starved and stimulated with insulin (100 ng/ml) for the indicated periods of time. Whole-cell lysates were analyzed by Western blotting with anti-IRS-1 antibody. (B) 3T3-L1 adipocytes infected with either control virus or small-t-antigen-encoding adenovirus (ST) were starved and stimulated with insulin for 3 min. Whole-cell lysates were prepared and immunoprecipitated (IP) with anti-IRS-1 antibody, followed by immunoblotting (IB) with anti-phosphotyrosine antibody (upper panel) or anti-p85 antibody (lower panel).