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. 2004 Oct;24(19):8778–8789. doi: 10.1128/MCB.24.19.8778-8789.2004

FIG. 5.

FIG. 5.

PKC λ phosphorylation and activity are enhanced in small-t-antigen-expressing 3T3-L1 adipocytes. (A) 3T3-L1 adipocytes were infected with either control virus or small-t-antigen-encoding adenovirus (ST), starved, and stimulated with insulin for 30 min. Whole-cell lysates were prepared and analyzed by Western blotting with phospho-specific PKC ζ/λ antibody (upper panel) or anti-PKC ζ/λ antibody (lower panel). (B) Data are shown as the percentage of PKC λ phosphorylation levels compared to that seen in maximally stimulated control cells and represent the mean ± SE of results from four independent experiments. (C) Cells were starved and stimulated with insulin for 30 min. Whole-cell lysates were immunoprecipitated with anti-PKC ζ/λ antibody and assayed for PKC λ activity as described in Materials and Methods. Data are presented as the increase (n-fold) in PKC λ activity compared to unstimulated control cells and represent the mean ± SE of results from three independent experiments.