JDE of GABA consisting of an edited condition (A, red) and a non-inverted reference (A, black) was performed using a semi-LASER sequence (voxel size 3×3×3 cm3, TR 3 s, TE 72 ms, 128 averages per JDE condition, acquisition time 13 min). The difference spectrum (B, scaling factor 1.1) exhibits expected co-edited glutamate and glutamine at 3.74 ppm and allows the isolation of the GABA 2CH2 signal at 3.01 ppm (dotted vertical line).