TABLE 1.
Primer sequences
| Primer | Sequencea |
|---|---|
| 1M | AGCTGCTCTAGAACAGCTATGACCATGATT |
| 2M | ACTGCAGGCAAGCTTAGATCAGACTGACTGCAGGGTTTTTGTTCCA |
| 3M | AGTCTGATCTAAGCTTGCCTGCAGTAACTTGTCGCGCCAATCGAG |
| 4M | TAGTGCTCTAGATTAGCTTCCTTAGCTCCT |
| 12-RSS Neg | TGGGCTGCAGGTCGACTGGCCATCTACAGACTGGAGCGGCCGCACTGCAGTCAGTCTGA |
| M13 Rev | AGGAAACAGCTATGACCATG |
| TN1 | GTAGTACTCCACTGTCTGGCTGTAGCGGCCGCCGCCTCGG GATCCTCTCA |
| TN2 | ACAGCCAGACAGTGGAGTACTACATGGCCAGGATCCCCGGGGATC |
| 12/12-1 | ATCGTGATCTAGAGATCCTGGCCAACAGCTATGACCATGACCATGATTACG |
| 12/12-2 | GGATCCCACAGTGGTAGTACTCCACACAAAAACCCTCGGGATC |
| 12/12-3 | GTGGAGTACTACCACTGTGGGATCC |
| 1m 23/23 | AGCTGCTCTAGATATGACCATGATTACGCC |
| 2m 23/23 | AGTACCATGGTTCCAGTCTGTAGCACTGTG |
| 3m 23/23 | CTACAGACTGGAACCATGGTACTAAAAAACCCTGCAGTCAGTCT |
| 4m 23/23 | TAGTGCTCTAGATCCTTAGCTCCTGAAAATC |
a
XbaI sites are underlined.