Figure 5.

Effect of pCQ on redistribution of the CXCR4 receptors under stimulation with CXCL12. (A) U2OS cells overexpressing EGFP-CXCR4 were treated with AMD3100 (300 nM) or pCQ16.7 (100 μM) for 30 min before exposing to CXCL12 (10 nM) for 1 h. The cells were then fixed and imaged by confocal microscopy (63×). (B) Cell surface expression of CXCR4 in U2OS cells measured by flow cytometry. Cells were treated as described above in Figure 4 (n=2).