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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: Shock. 2017 Jan;47(1):22–32. doi: 10.1097/SHK.0000000000000713

Fig. 7. Depletion of regulatory T cells exacerbates the development of experimental necrotizing enterocolitis by decreasing the proliferative capacity of crypt-based intestinal stem cells.

Fig. 7

A, the proliferative capacity of crypt-based ISCs was assessed by Ki67 staining of terminal ileum sections obtained from wild-type C57Bl/6 and Foxp3+DTR mice submitted to experimental NEC (ii, vi and iv, viii respectively) or breast-fed controls (i, v and iii, vii respectively). The outlined areas in i – iv are shown at a higher magnification in v – viii. Proliferating cells are indicated by arrows and demonstrate a significant decrease of dividing cells within the crypts of mice submitted to NEC. B, Ki67 staining was quantified as described in the methods section and expressed as a percentage of the total number of cells identified within the crypts per high-magnification field. Scale bar = 50μm. Data depicted is presented as mean ± SD and represents 3 independent experiments with at least 10 mice per group. ***P ≤ 0.0001 determined by ANOVA followed by Tukey's multiple comparisons.