Table 1. Nucleotide sequences designed as forward and reverse primers of each specific gene.
Gene name | Forward & reverse primer sequence |
---|---|
Adiponectin | |
Forward | 5' CCGCTTACATGTATCACTC 3' |
Reverse | 5' ATACTGGTCGTAGGTGAAGA 3' |
CD68 | |
Forward | 5' AAGTCCTAGTCCAAGCTCTA 3' |
Reverse | 5' AGGACACATTGTATTCCACT 3' |
CD14 | |
Forward | 5' CTCAGAATCTACCGACCA 3' |
Reverse | 5' ATAGATTGAGCGAGTTTAGC 3' |
IL-6 | |
Forward | 5' GGAGTTTGTGAAGAACAACT 3' |
Reverse | 5' CTAGGGTTTCAGTATTGCTC 3' |
Leptin | |
Forward | 5' CTGTCGTGACTGACTCTATG 3' |
Reverse | 5' GCTAAGTGATTTCTCATTCC 3' |
TNF-α | |
Forward | 5' GAACACCCTGGTACTAACTC 3' |
Reverse | 5' TAGATAAGGTACAGCCCATC 3' |
IL-6, interleukin-6; TNF-α, tumor necrosis factor α.