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. 2016 Dec 20;10:287. doi: 10.3389/fncel.2016.00287

Figure 3.

Figure 3

Ac-K123 SOD1 localization within granule, basket and stellate cells (SC) somata and ML neurites of the cerebellum. (A) Sagittal view micrograph montage (scale bar, 500 μm) of cerebellum showing Ac-K123 SOD1 (R26) immunostaining within the cerebellar cortex and underlying arbor vitae (Arb). The cerebellar cortex GCL and ML are noted. (B) Confocal micrograph (scale bar, 50 μm) demonstrating Ac-K123 SOD1 (R26) immunostaining in the cerebellum. Shown is the cerebellar cortex layers, consisting of the GCL, Purkinje cell layer (PCL) and ML. Inset shows a 2× zoom of the boxed region demonstrating Ac-K123 SOD1 labeled processes within the ML (arrow). (C) Higher magnification confocal micrograph (scale bar, 20 μm) of GCL, PCL and ML immunostained for Ac-K123 SOD1 (R26) and calbindin D28K with nuclear counterstaining by Hoechst 33342. (D) Confocal micrograph (scale bar, 20 μm) of GCL, PCL, and ML immunostained for Ac-K123 SOD1 (R26) and Tuj1. Examples of SC and basket cells (BC) are marked by arrows.