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. 2016 Dec 20;9:135. doi: 10.3389/fnmol.2016.00135

Figure 1.

Figure 1

Culture and identification of neural stem cells (NSCs). (A) Cell morphology and nestin expressions. (a–c) Immunostaining of the Nestin (green) in neurosphere before seeded on substrates. (d–f) Immunostaining of the Nestin (green) in neurosphere after seeded on PLL. Cell nuclei was stained with 4′,6-diamidino-2-phenylindole (DAPI; blue). (B) Representative immunofluorescence images of the cultures at day 7, as one characteristic of NSCs, the cells differentiated into neurons and astrocytes. (a) Immunostaining of the β-tubulin III (green) of the cultures. (b) Immunostaining of the glial fibrillary acidic protein (GFAP; red) of the cultures. (c) Cell nuclei was stained with DAPI (blue). (d) Merge picture of (a–c). Scale bar = 25 μm.