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. 2016 Dec 20;9:135. doi: 10.3389/fnmol.2016.00135

Figure 5.

Figure 5

NSC differentiation on day 7 of culture. (A) Representative immunofluorescence images of NSCs differentiated neurons and astrocytes in the four groups. Cells were stained for β-tubulin III (green), GFAP (red) and DAPI for the nucleus (blue). Scale bar = 25 μm. (B) Quantitative analysis of β-tubulin III and GFAP staining in the experimental groups. Dates are the mean ± SEM of three independent experiments (n = 6 for each group); **P < 0.01 vs. control; ▲▲P < 0.01 vs. ketamine; ΔΔP < 0.01 vs. ketamine plus MC. (C) Representative images of β-tubulin III and GFAP expression by western blot in the four groups. (D) Quantitative analysis of β-tubulin III and GFAP expressions normalized to β-actin. Data were expressed as the ratio to optical density (OD) values of the corresponding controls and showed as the mean ± SEM of three independent experiments (n = 6 for each group); **P < 0.01 vs. control; ▲▲P < 0.01 vs. ketamine; ΔΔP < 0.01 vs. ketamine plus MC.