Figure 7.

MC reverses the inhibitory effect of ketamine by PI3K/Akt pathway. (A,B) MC time-dependently prevented ketamine-evoked decrease of p-Akt. (A) Representative images of p-Akt, Akt and β-actin expression by western blot in the four groups. (B) Quantitative analysis of p-Akt, Akt and β-actin expressions normalized to β-actin. (C,D) MC time-dependently prevented ketamine-evoked decrease of p-Gsk-3β. (C) Representative images of p-Gsk-3β, glycogen synthase kinase-3beta (Gsk-3β) and β-actin expression by western blot in the four groups. (D) Quantitative analysis of p-Gsk-3β, Gsk-3β and β-actin expressions normalized to β-actin. Data were expressed as the ratio to OD values of the corresponding controls and showed as the mean ± SEM of three independent experiments (n = 6 for each group); **P < 0.01 vs. control; ^▲▲P < 0.01 vs. ketamine; ^ΔΔP < 0.01 vs. ketamine plus MC.