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. 2016 Dec 20;7:1899. doi: 10.3389/fpls.2016.01899

Figure 1.

Figure 1

Cell wall invertase activity and gene expression in different Arabidopsis organs. (A) CWIN activity in Col-0 and cwin1-1 was assayed from frozen ground tissues of 5–6 week-old soil-grown plants (leaves and roots) or 9-day-old seedling roots. Data represent mean (±SE) of 2 (seedling roots) or 3 (leaves and roots) independent experiments. (B,C) Relative gene expression of AtCWIN1, AtCWIN5 (B), AtCIF1 and AtC/VIF2 (C) genes was performed by RT-qPCR and results were normalized to the plant reference genes At4g26410 and AtACTIN2 (At3g18780). Data represent mean (±SE) of 3 independent experiments. AtCWIN2 and -4 are not presented because transcript levels were below the detection threshold. For (A), asterisks represent significant differences between organs of Col-0 plants (Student's t-test, *P < 0.05; **P < 0.01).