Figure 6.

Intracellular invertase activity and AtVINs gene expression analysis upon B. cinerea infection. (A) VIN and CIN activities in Mock and B. cinerea (5 × 10⁴ conidia ml⁻¹) treated leaves of Col-0 plants. Data represent mean (±SE) of at least 3 independent experiments. (B) Expression of AtVIN genes in Mock and B. cinerea (5 × 10⁴ conidia ml⁻¹) treated leaves of Col-0 plants. Relative gene expression was performed by RT-qPCR and results were normalized to the plant reference genes At4g26410 and AtACTIN2 (At3g18780). Data represent mean at least 3 independent experiments. (C) VIN activity in Mock and B. cinerea (5 × 10⁴ conidia ml⁻¹) treated leaves of Col-0 and cwin1-1 plants. Data represent mean (±SE) of 3 independent experiments. (A,C) are the results of two sets of experiments. Asterisks represent significant differences compared to the corresponding Mock condition (Student's t-test, ^*P < 0.05; ^**P < 0.01).