Figure 8.

B. cinerea invertase activity, sugar transport and gene expression (A) Cell wall and intracellular (acidic and neutral) invertase activities in B. cinerea mycelium grown in vitro (mean +/− SE of 3 independent experiments). (B) [¹⁴C]-sucrose uptake activity in B. cinerea mycelium grown in vitro (mean +/− SE of 3 independent experiments). Active sucrose uptake results from the difference between total and CCCP-insensitive uptakes. (C) Inhibition of [¹⁴C]-sucrose uptake activity by sucrose, glucose:fructose (1:1 mixture) and mannitol supplied in 20-fold excess in B. cinerea mycelium grown in vitro (mean +/− SE of at least 2 independent experiments). (D) Relative expression of B. cinerea putative invertase (Bc1g10247 and Bc1g16010), fructose (BcFRT1) and hexoses transporter (Hxt1-17) genes in infected Arabidopsis leaves (5 × 10⁴ conidia ml⁻¹). Gene expression analysis was performed by RT-qPCR and results were normalized to the B. cinerea TUBA gene. Data represent mean (±SE) of 3 independent experiments.